(A) Western blot assay showing the effects of overexpression of Notch1 and Jagged1 in vivo. (B) Western blot assay showing the effects of knockdown of Notch1 and Jagged1 expression in vivo. (C) Immunofluorescence assay showing the fluorescence intensity of CD31 labeling in the Notch signaling pathway activation and inhibition groups. The fluorescence intensity of CD31 is presented as a ratio to the control group. Data are expressed as the mean ± SD (n = 5 in each group). Differences between groups were compared using a one-way analysis of variance and a nonparametric Mann-Whitney U test. †P < 0.05, vs. vector group. (D) Effects of Notch signaling pathway activation and inhibition in rats with traumatic brain injury. Activation of the Notch signaling pathway promotes neurovascular repair after traumatic brain injury: Ran QS, Yu YH, Fu XH, Wen YC - Neural regeneration research (2015). Not altered. CC.