Schematic representation of the new AuNP-based system for colorimetric detection of PAX1 gene DNA methylation.Notes: (A) Sodium bisulfite conversion of unmethylated cytosines into uracil while methylated cytosines remain unconverted. (B) PCR amplified with thiol-labeled methylation specific primer of PAX1. (C) Aggregation of AuNPs in unmethylated DNA sample and the color of AuNP colloid solution changed from red to purple-gray, whereas no aggregation in methylated DNA sample and no color change were observed in AuNPs colloid solution upon salt adition.Abbreviations: AuNPs, gold nanoparticles; PCR, polymerase chain reaction. Real-time colorimetric detection of DNA methylation of the PAX1 gene in cervical scrapings for cervical cancer screening with thiol-labeled PCR primers and gold nanoparticles. International Journal of Nanomedicine. Not Altered. CC.
Cervical cancer screening and prevention is done by:
Pap smears which is a method of cervical screening used to detect potentially precancerous and cancerous processes in the cervix. It involves collecting cells from your cervix.
Gardasil is an HPV vaccine approved by the U.S. FDA, and can be used for both girls and boys. This vaccine can prevent most cases of cervical cancer if the vaccine is given before girls or women are exposed to the virus.
