Confirmation and identification of adenocarcinoma in the lung and pleural effusions with subsequent coexpressions of EMT-associated markers.(A) Immunohistochemical identification of adenocarcinoma cells showed positive results of CK-7 (arrows) in both primary pulmonary tumor (Magnification, 200×) and cell nests in cell block of MPE at lower and higher magnifications (a, b) (Magnification, 40× and 400×); Double-staining of TTF-1/CEA revealed that TTF-1 (brown color, arrow heads) and CEA (red color, arrows) were evenly positive-stained in primary pulmonary tumor (c) (Magnification, 200×), while CEA (+) cells (arrows) are stained stronger than those of TTF-1 (+) cells (arrow heads) in cell block of MPE at lower and higher magnifications (d). (Magnification, 40× and 400×). (B) Double-staining of E-cadherin/vimentin showed that decreased E-cadherin (brown color, arrows) and increased vimentin (red color, arrow heads) expressions were found at the invasive fronts of primary pulmonary tumor and in cell block of MPE. (Magnification, 200×) (C). Pulmonary Adenocarcinoma in Malignant Pleural Effusion Enriches Cancer Stem Cell Properties during Metastatic Cascade. Chen SF, Lin YS, Jao SW, Chang YC, Liu CL, Lin YJ, Nieh S - PloS one (2013). Not Altered. CC.