(A) Plot of the means of the phenotypic trajectories of all 154 compounds in two highly informative dimensions, “Cell Damage” and “DNA Damage”. Topoisomerase inhibitors (red) and antimetabolites (magenta) have been highlighted. (B) The plot of phenotypic trajectory means in the “Cell Damage” and “Tubulin Intensity” dimensions. Microtubule stabilizers (yellow), microtubule inhibitors (purple), and HSP90 inhibitors (green) have been highlighted. (C) The plot of phenotypic trajectory means in the “Cell Damage” and “Blebbishness” dimensions. Proteasome inhibitors (blue) and CDK inhibitors (teal) have been highlighted. (D) The plot of phenotypic trajectory means in the “Cell Damage” and “Structural Damage” dimensions. HDAC inhibitors (dark orange) and CDK inhibitors (teal) have been highlighted. (E) Example microscopy image of untreated cells. The red channel depicts α-tubulin intensity, the green channel Hoescht stain intensity, and the blue channel p-H2A.X stain intensity. (F–T) Example microscopy images of cells treated by 15 different compounds. Robust Classification of Small-Molecule Mechanism of Action Using a Minimalist High-Content Microscopy Screen and Multidimensional Phenotypic Trajectory Analysis: Twarog NR, Low JA, Currier DG, Miller G, Chen T, Shelat AA - PloS one (2016). Not altered. CC.