Mitochondrial Function. Analysis of mitochondrial function A: Immunofluorescence observation of mitochondria localization. The mitochondria of OVCAR8 cells and NCI_ADR/RES cells were detected with mitochondrial ATP synthase β antibody and CF™488A labeled fluorescent secondary antibody (green). Nuclei were stained with DAPI (blue). Magnification: all images 1200×. B: Fluorescence images of Mitotracker Green staining in OVCAR8and NCI_ADR/RES cells. Mitochondria in the cells were stained with 50 nM Mitotracker Green for 20 min and observed under a confocal microscope. C: Mitochondria membrane potential analysis in OVCAR8 and NCI_ADR/RES cells. Cells were stained with 50 nM Rhodamine B for 30 min and analyzed with a flow cytometer.Quantitative proteomics analysis identifies mitochondria as therapeutic targets of multidrug-resistance in ovarian cancer. Chen X, Wei S, Ma Y, Lu J, Niu G, Xue Y, Chen X, Yang F - Theranostics (2014). Not Altered. CC.