Receptor Mediated Endocytosis. In vitro internalization of ILNPs.The SPIO-loaded ILNPs were observed internalized near the cell membrane, mediated by clathrin-dependent pinocytosis or receptor-mediated endocytosis (b), macropinocytosis (h and e), clathrin-independent pinocytosis (d) or phagocytosis (g). SPIO-loaded ILNPs were also observed in the gaps between cells (f), in the macropinosomes mediated by macropinocytosis (a), in the phagosomes mediated by phagocytosis (i) and in the endosomes (c). Ligand modified nanoparticles increases cell uptake, alters endocytosis and elevates glioma distribution and internalization. Gao H, Yang Z, Zhang S, Cao S, Shen S, Pang Z, Jiang X - Scientific reports (2013). Not Altered. CC.